Hybridization of leucyl-transfer ribonucleic Acid isoacceptors from green leaves with nuclear and chloroplast deoxyribonucleic Acid.
نویسندگان
چکیده
Chromatographically distinct isoacceptors of leucyl-tRNA from mature bean leaves were all observed to hybridize with bean chloroplast and nuclear DNA in a ratio similar to that exhibited by the unfractionated leucyl-tRNA. Under the same hybridization conditions, maize tRNA failed to form a stable hybrid with bean DNA, and levels of hybridization between bean-leaf leucyl-tRNA and nuclear or chloroplast DNAs from tobacco and maize were relatively small.
منابع مشابه
Variations in the Levels of Chloroplast tRNAs and Aminoacyl-tRNA Synthetases in Senescing Leaves of Phaseolus vulgaris.
The relative amounts of chloroplast tRNAs(Leu), tRNA(Glu), tRNA(Phe), tRNAs(Thr), and tRNA(Tyr) and of chloroplastic and cytoplasmic aminoacyl-tRNA synthetases were compared in green leaves, yellowing senescing leaves, and N(6)-benzyladenine-treated senescing leaves from bean (Phaseolus vulgaris, var Contender). Aminoacylation of the tRNAs using Escherichia coli aminoacyl-tRNA synthetases indic...
متن کاملFactors affecting the release of nuclear ribonucleic acid from the nucleus in vitro.
According to a widely accepted view, the structure of chromosomal deoxyribonucleic acid in the nucleus determines the enzymatic composition of the cytoplasm. An attractive proposition which would explain this genetic control of cytoplasmic enzymes is that deoxyribonucleic acid in some way determines the structure of nuclear ribonucleic acid, that the nuclear ribonucleic acid passes out into the...
متن کاملChloroplast DNA codes for transfer RNA . Jan
Transfer RNA's were isolated from Euglena gracilis. Chloroplast cistrons for tRNA were quantitated by hybridizing tRNA to ct DNA. Species of tRNA hybridizing to ct DNA were partially purified by hybridization-chromatography. The tRNA's hybridizing to ct DNA and nuclear DNA appear to be different. Total cellular tRNA was hybridized to ct DNA to an equivalent of approximately 25 cistrons. The tot...
متن کاملDifferent fates of the chloroplast tufA gene following its transfer to the nucleus in green algae.
Previous work suggested that the tufA gene, encoding protein synthesis elongation factor Tu, was transferred from the chloroplast to the nucleus within the green algal lineage giving rise to land plants. In this report we investigate the timing and mode of transfer by examining chloroplast and nuclear DNA from the three major classes of green algae, with emphasis on the class Charophyceae, the ...
متن کاملDeoxyribonucleic acid-ribonucleic acid hybridization studies on the L-Arabinose operon of Escherichia coli B-r.
An increase in the rate of synthesis of ara-specific messenger ribonucleic acid as measured by deoxyribonucleic acid-ribonucleic acid hybridization has been detected in the induced wild-type (ara(+)) strain of Escherichia coli B/r as compared with the uninduced control, thus providing evidence that regulation of the positively controlled l-arabinose operon is at the level of transcription.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 70 12 Pt 1-2 شماره
صفحات -
تاریخ انتشار 1973